Characterization of trypanosome evansi antigen by SDS-page and western blot

SDS-PAGE of T.evansi antigen after staining with commassie blue stain showed the presence of 26 bands ranging between 222.56 - 9.0 KDa. Western blot analysis revealed immunoprecipitation only with 2 proteins components with camels positive serum and had molecular weight of 98.976 and 16.678 KDa. While with mice positive sera revealed only one immuoprecipitation with protein component had molecular weight 16.678 KDa

Incidence of theileriosis, babesiosis and anaplasmosis in cattle in Tripoli - Libya

Bovine theileriosis, babesiosis and anaplasmosis are a haemoparasitic disease caused by protozoans of Genus Theileria, Babesia and Anaplasma respectively. These haemoparasites are responsible for considerable losses due to mortality, weight loss and a reduction in milk beside the cost of prophylactic measures. The results showed that 51 [11.7%] and 13[2.9%] out of 443 cattle from different localities in Tripoli-Libya by Giemsa stained blood and lymph node smears were infected only with Theileria species respectively. The incidence of Theileria species by Giemsa stained blood films examination in different localities in Tripoli was 0.0%, 22.7% and 35.4% in Engeela, Algyran and Fum-Mulgha respectively. While the total incidence of T.mutans, B.bigemina and A.marginale in the examined cattle by different ELISA kits were 50.6%, 12.9, and 3.4%, respectively. Also, the present work recorded mixed infection